Radioimmunoassays for Rat Follicle Stimulating and Luteinizing Hormones
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چکیده
منابع مشابه
Developmental Patterns of Follicle-stimulating, Luteinizing and Thyroid-stimulating Hormones in the Hypothyroid Female Rat
The developmental patterns of plasma and pituitary levels of the three glycoprotein hormones, FSH, LH and TSH, were studied in hypothyroidism and after thyroxine administration. Rats were radiothyroidectomized at birth and the pituitary and circulating hormone levels were measured at five-day intervals from 5 to 4o days of age by double-antibody radioimmunoassay. Body weight and weight of selec...
متن کاملLuteinizing and follicle-stimulating hormones. Cell-free translations of messenger RNAs coding for subunit precursors.
Polyadenylated RNA prepared from pituitary glands of normal and castrate rats was translated in membrane-free heterologous cell-free systems derived from wheat germ and rabbit reticulocytes. Sodium dodecyl sufate (SDS)-polyacrylamide gel electrophoresis and autoradiography demonstrated a major [35S]methionine-labeled translation product of castrate pituitary mRNA with apparent Mr = 14,000. This...
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Background and Aims: Vitamin D has antioxidant and anti-inflammatory activities. The present study aimed to investigate the protective effect of vitamin D on Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), testosterone hormones and testicular tissue changes induced by methotrexate in adult male rats. Materials and Methods: In this experimental study, 30 adult male Wistar rats wei...
متن کاملSerum insulin, glucose and non esterified fatty acids after administration of follicle-stimulating and luteinizing hormones in bitches.
This paper reports the effect of the simultaneous administration of follicle-stimulating (FSH) and luteinizing hormones (LH) on serum glucose, insulin and nonesterified fatty acid responses after glucose or insulin challenge. The animals were originally at anestrous. FSH (dose 2.5 U/kg body wt.) and LH (0.27 U/kg body wt.) were s.c. injected on days 1, 4, 8 and 11. Vaginal smears were obtained ...
متن کاملDissociation of human follicle-stimulating hormone. Comparison with luteinizing hormone.
Rat testis tissue receptor assays were utilized to study the kinetics of dissociation of human follicle-stimulating hormone (hFSH) and luteinizing hormone (hLH) under varying conditions of urea concentration and pH. In these competitive protein binding assays, 125I-hFSH and 125I-hLH were the radioligands and hormone dissociation was followed by a decrease in the ability of the dissociating horm...
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ژورنال
عنوان ژورنال: Endocrinologia Japonica
سال: 1971
ISSN: 0013-7219,2185-6370
DOI: 10.1507/endocrj1954.18.477